Methylthioninium chloride · 100 mL of 30% w/v aqueous solution- ·100 mL amber glass bottle, 30% w/v solution
Methylene Blue 100 mL / 30%
Phenothiazine dye — research-grade 30% aqueous solution.
Methylene blue is a phenothiazine dye with extensive applications in cellular and mitochondrial research, histology, and redox biology.
What the peptide is.
Methylene blue (methylthioninium chloride) is one of the oldest synthetic dyes, first synthesised in 1876. Clinically approved for methemoglobinemia (IV administration) but widely used as a research reagent in cellular biology, mitochondrial-function studies, and neuroprotection research.
Omega Grade supplies a 30% w/v aqueous research-grade solution. Not the pharmaceutical-grade IV formulation.
Electron-cycling between oxidised (MB) and reduced (leucoMB) forms. Mitochondrial complex IV alternate electron acceptor; ROS-scavenging activity; NO-synthase inhibition.
Reconstitution, storage, and handling.
Lab-technical guidance for the researcher handling the material. Not therapeutic dosing, not administration guidance — those are not within the scope of this supply.
- Recommended solvent
- N/A — pre-formulated solution
- Typical volume
- N/A
- 30% w/v aqueous solution in amber glass. Protect from light.
- Lyophilised
- Room temperature or 2–8 °C, amber glass, sealed.
- Reconstituted
- N/A — pre-formulated.
- Shelf life · sealed
- 24 months at room temperature.
- Shelf life · reconstituted
- N/A
- ▸Strong blue colour — stains surfaces and skin on contact. Use gloves.
This lot, documented.
Every shipment is accompanied by a signed four-page analytical report including the HPLC chromatogram, ESI-MS spectrum, purity calculation, and sample handling chain-of-custody.
The report you will receive is specific to the lot dispatched to you. The display below is a current representative example.
04:22:11▸lot 26-04-A received ┈ 5.00 g04:22:40▸weight check ┈ PASS ±0.4 mg04:23:12▸sample → janoshik ┈ 50 mg04:23:12▸tracking #jnk-921833405:18:03▸HPLC result ┈ 99.24 %05:18:04▸MS obs 4729.42 Da ┈ PASS05:18:05▸coa.pdf signed ┈ attached05:18:06▸lot approved ┈ LIVE
REPORT ID JA-26-04-012
SUBJECT Methylene Blue 100 mL / 30%
DESIGNATION Methylthioninium chloride
LOT 26-04-A
METHOD RP-HPLC, ESI-MS
COLUMN C18, 250 x 4.6 mm, 5 um
MOBILE PHASE A: 0.1% TFA in H2O
B: 0.1% TFA in ACN
GRADIENT 5 -> 65% B over 20 min
FLOW RATE 1.0 mL/min
DETECTION UV 214 nm
INJECTION 20 uL @ 0.5 mg/mL
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PURITY (A%) 99.00
MASS EXP. 319.85 Da
MASS OBS. 319.87 Da
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CONCLUSION Identity CONFIRMED · Purity PASS
SIGNED BY J. Novak, MSc · Janoshik Analytical SVK
DATE 2026-04-11Research references for Methylene Blue 100 mL / 30%.
1 peer-reviewed publications. Links resolve to PubMed. DOIs are provided where available.
- [01]Rojas JC, Bruchey AK, Gonzalez-Lima F
Neurometabolic mechanisms for memory enhancement and neuroprotection of methylene blue
Progress in Neurobiology · 2012
- Q.Why is this in a peptide catalogue?
- A.Methylene blue is commonly paired with mitochondrial peptides in bioenergetics and neuroprotection research. It is not a peptide — it is a phenothiazine dye.
This peptide is supplied by Omega Grade for in vitro laboratory research use. It is not a drug, food, cosmetic, or dietary supplement. It is not approved by the FDA, EMA, MHRA, PMDA, or any other regulatory authority for any therapeutic indication.
No claim of therapeutic benefit is made or implied. The material is not intended for human or veterinary consumption, administration, or any form of self-experimentation. By purchasing, you acknowledge the researcher terms and accept full responsibility for local import compliance and all handling.
