TB-500 10 mg
Also known as · Thymosin β-4 · Tβ4 · Timbetasin
Synthetic full-length 43-residue thymosin β-4. The most abundant intracellular actin-sequestering peptide in mammalian tissues.
Chemistry quick-facts
- CAS
- 77591-33-4
- Formula
- C212H350N56O78S
- MW
- 4963.4 Da
- Length
- 43 aa
- Status
- Limited clinical evidence. Thymosin β-4 has been investigated in Phase 2 trials for dry-eye disease (RGN-259), pressure ulcers, and epidermolysis bullosa. No approval for therapeutic use.
- Purity (HPLC)
- ≥ 99.1%
Mechanism of action
G-actin sequestration → cytoskeletal dynamics. Secondary: integrin-linked kinase upregulation, laminin-5 expression modulation.
Research context
TB-500 denotes synthetic thymosin β-4 (Tβ4), a naturally occurring 43-amino-acid peptide. It is among the most abundant intracellular proteins, functioning primarily as a G-actin sequestering molecule that regulates actin polymerisation and cell motility.
RegeneRx Biopharmaceuticals has developed synthetic Tβ4 under the code RGN-259 through Phase 2 trials in dry-eye disease and neurotrophic keratopathy.
Published studies
A non-exhaustive list of peer-reviewed studies referenced when curating this entry. Click through to the primary source for the full text.
- Goldstein AL, Hannappel E, Sosne G, Kleinman HK (2012). Thymosin β4: a multi-functional regenerative peptide. Expert Opinion on Biological Therapy.
- Sosne G, Ousler GW (2015). Thymosin beta 4 ophthalmic solution for dry eye — Phase 2. Clinical Ophthalmology.
Active clinical trials
- NCT02623244Phase 3 · Completed
ARISE-3: RGN-259 in dry eye
Reconstitution
- Solvent
- Bacteriostatic water for injection (0.9% benzyl alcohol) — included with kit
- Typical volume
- 1 – 2 mL
- · Inject diluent slowly down the side of the vial onto the cake.
- · Swirl gently — do not shake.
- · Solution should be clear and colourless. Discard if not.
Storage
- Lyophilised
- −20 °C, desiccated, protected from light. Stable at 15–25 °C for up to 30 days in transit.
- Reconstituted
- 2–8 °C, original vial, consumed within 4 weeks.
- Shelf life — sealed
- 24 months from date of manufacture when stored lyophilised at −20 °C.
FAQ
Is this the same as the short Ac-SDKP tetrapeptide?+
No. Full 43-residue Tβ4. Ac-SDKP (goralatide) is a separate N-terminal tetrapeptide with distinct activity.
This page summarises the research literature on TB-500 10 mg for in vitro laboratory investigation only. It is not medical advice, not a treatment recommendation, and not a substitute for a qualified physician. Material is sold for analytical chemistry and cell-culture research, not for human or veterinary administration.
- ResearchBPC-157 + TB-500 10/10 mg kit
The most-requested regenerative-peptide kit in the catalogue. BPC-157 and thymosin β-4 combined in a single lyophilised vial — commonly paired in prec
- ResearchBPC-157 15 mg
15-residue synthetic pentadecapeptide. Most extensively studied in rodent tendon, ligament, skin, and gastric-mucosal models.
- ResearchBPC-157 + TB-500 20/20 mg pen
High-dose pen format of the BPC-157 + TB-500 blend. Double the dose of the standard vial kit, in a pre-filled pen device.
- ComparisonBPC-157 vs TB-500 — research differences and combined-use literature
BPC-157 and TB-500 (thymosin beta-4 fragment) are the two most-studied research peptides in tissue-repair literature. Their mechanisms differ — what the research shows, and why they're often paired in protocol papers.
- Biomarkerhs-CRP 2.5 mg/L
High-sensitivity C-reactive protein at 2.5 mg/L sits in the 'intermediate cardiovascular risk' category most cardiology research uses. What it measures, what drives it, and how to interpret your number.
- ComparisonBPC-157 acetate vs arginate — salt forms compared
BPC-157 is sold as either the acetate or arginate salt. The arginate form has improved stability across pH variations; acetate is the historical default.