§ Research summary · Nootropic

Methylene Blue 100 mL / 30%

Also known as · Methylthioninium chloride

Nootropic·1 min read·reviewed 2026-05-07

Methylene blue is a phenothiazine dye with extensive applications in cellular and mitochondrial research, histology, and redox biology.

Chemistry quick-facts

CAS
61-73-4
Formula
C16H18ClN3S
MW
319.85 Da
Status
Approved for methemoglobinemia treatment (IV, pharmaceutical grade only). Research-grade methylene blue is used extensively in cell biology, mitochondrial research, and neuroprotection studies.
Purity (HPLC)
≥ 99.0%

Mechanism of action

Electron-cycling between oxidised (MB) and reduced (leucoMB) forms. Mitochondrial complex IV alternate electron acceptor; ROS-scavenging activity; NO-synthase inhibition.

Research context

Methylene blue (methylthioninium chloride) is one of the oldest synthetic dyes, first synthesised in 1876. Clinically approved for methemoglobinemia (IV administration) but widely used as a research reagent in cellular biology, mitochondrial-function studies, and neuroprotection research.

Omega Grade supplies a 30% w/v aqueous research-grade solution. Not the pharmaceutical-grade IV formulation.

Published studies

A non-exhaustive list of peer-reviewed studies referenced when curating this entry. Click through to the primary source for the full text.

  1. Rojas JC, Bruchey AK, Gonzalez-Lima F (2012). Neurometabolic mechanisms for memory enhancement and neuroprotection of methylene blue. Progress in Neurobiology.

Reconstitution

Solvent
N/A — pre-formulated solution
Typical volume
N/A
  • · 30% w/v aqueous solution in amber glass. Protect from light.

Storage

Lyophilised
Room temperature or 2–8 °C, amber glass, sealed.
Reconstituted
N/A — pre-formulated.
Shelf life — sealed
24 months at room temperature.

FAQ

Why is this in a peptide catalogue?+

Methylene blue is commonly paired with mitochondrial peptides in bioenergetics and neuroprotection research. It is not a peptide — it is a phenothiazine dye.

Research-use disclaimer

This page summarises the research literature on Methylene Blue 100 mL / 30% for in vitro laboratory investigation only. It is not medical advice, not a treatment recommendation, and not a substitute for a qualified physician. Material is sold for analytical chemistry and cell-culture research, not for human or veterinary administration.

View Methylene Blue 100 mL / 30% in the catalogue →
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